Journal de la technologie microbienne et biochimique

Abstrait

Changes in the Hydrophobic Proteins in Response to Biotin Administration in Serum of Infant Patients with Alopecia as Assessed by the Protein Micro-Sequencing Method

Kiyomi Abe, Kou Hayakawa, Kenji Ihara, Kentaro Deguchi and Takeaki Nagamine

Introduction: In order to clarify the mechanism of hair growth induced by biotin, we compared the serum proteins before and after the oral biotin administration.

Materials and methods: The serum of three biotin-deficient children with alopecia were studied. Oral biotin administration was performed. Component proteins of serum were studied by using unique quantitative proteinmicrosequencing- deciphering method.

Results: Hydrophobic membrane-proteins of ultra-high-sulphur keratin-associated protein was detected in the serum of biotin-deficient children with alopecia. This membrane protein in the serum disappeared after the biotin administration. This phenomenon is significant (p<0.05; Mann-Whitney’s U test).

Conclusion: Biotin deficiency in children induced the excretion of membrane proteins into the blood, and biotin administration prevented this phenomenon. Therefore, biotin was expected to regulate the expression of membrane proteins.