Journal de la nanomédecine et de la nanotechnologie

Abstrait

Formulation, Optimization and Dissolution of Piper Nigrum Nanosuspension.

Ume Habiba1, Muhammad Tahir Khan1, Sumaira Sharif1, Madeeha Shahzad Lodhi1, Adnan Ali2, Memoona Rehman3, Muhammad Aamar Javed4, Fatiqa Zafar

The aim of present study was to enhance the dissolution rate of Piper nigrum, plant extract by formulating its nanosuspension. Piperine which is the main bioactive constituent of Piper nigrum is responsible for its bioactivities. Nanoprecipitation approach was used for the formulation of nanosuspension. The present research was divided into different phases. The first phase of this study was consisting of formulation and optimization of formulative parameters for the preparation of a stable nanosuspension of Piper nigrum. Nanosuspension formulation was prepared by using sodium lauryl sulphate, hydroxy propyl methyl cellulose, poly vinyl alcohol and polysorbate 80. The formulated nanosuspension was further accessed to identify particle size, Polydispersity index, and zeta potential value. Characterization and stability studies of optimized Nanosuspension were the next phase. The formulated nanosuspension was characterized by using scanning electron microscope. Storage stability of formulated nanosuspension was determined after three months of storage at room temperature and in the refrigerated condition. In vitro dissolution studies were performed for coarse plant extract and nanosuspension at different pH values (6.8, 7.0, 7.2). Antioxidant, antimicrobial and antifungal activities of optimized nanosuspension were also determined with respect to coarse plant extract. Results of formulated nanosuspension showed that HPMC is the suitable stabilizer for the Piper nigrum nanosuspension. The mean particle size of Piper nigrum nanosuspension is 341.0nm with polydispersity index of 0.190. Piper nigrum showed improved results of dissolution rate for nanosuspension as compared to its coarse suspension at pH 7.0. Results of all bioactivities illustrated better potential of nanosuspension as compared to coarse plant extract. In conclusion, our study showed that the nanotechnology can be used as an effective approach to enhance the dissolution rate of Piper nigrum nanosuspension.