Indexé dans
- Ouvrir la porte J
- Genamics JournalSeek
- Clés académiques
- JournalTOCs
- Infrastructure nationale des connaissances en Chine (CNKI)
- Répertoire des périodiques d'Ulrich
- RechercheRef
- Université Hamdard
- EBSCO AZ
- Répertoire d'indexation des résumés pour les revues
- OCLC - WorldCat
- Publions
- Fondation genevoise pour la formation et la recherche médicales
- Pub européen
- Google Scholar
Liens utiles
Partager cette page
Dépliant de journal
Revues en libre accès
- Agriculture et aquaculture
- Alimentation et nutrition
- Biochimie
- Bioinformatique et biologie des systèmes
- Business & Management
- Chimie
- Génétique et biologie moléculaire
- Immunologie & Microbiologie
- Ingénierie
- La science des matériaux
- Neurosciences & Psychologie
- Science générale
- Sciences cliniques
- Sciences environnementales
- Sciences médicales
- Sciences pharmaceutiques
- Sciences vétérinaires
- Soins infirmiers et soins de santé
Abstrait
Induction of immune tolerance towards allogeneic cells using fetal directed placental injection in a murine model
Yukiko Shimazu, Masayuki Endo, Katsuto Tamai, Kei Takahashi, Takekazu Miyoshi, Hiroshi Hosoda, Alan W. Flake, Tadashi Kimura, Jun Yoshimatsu
Objective: In utero exposure to foreign antigens prior to the development of the immune system induces immune tolerance. We aimed to induce immune tolerance towards allogeneic cells by early gestational transplacental injection under ultrasound guidance in a murine model. Methods: Bone marrow cells from C57BL/6-Green Fluorescence Protein transgenic mice were transplanted into the placenta of Balb/c fetal mice at 11-day of gestation under ultrasound guidance. Each fetus was injected with 2 × 105 cells/2.5 μl. After birth, we evaluated the immune response against allogeneic donor cells. Results: The birth survival rate was 21.2% for allogeneic mice. Survival of the donor skin graft was 75% and successful in mice injected with fetal transplacental cells, whereas the transplanted allogeneic skin was all rejected within 4 weeks in control naïve mice (p=0.007). Cytotoxic immune reactivity against the allogeneic cells was suppressed according to the ELISPOT assay (p=0.002). Conclusion: We showed that early transplacental allogeneic cell injection can induce donor-specific tolerance sufficient to allow tissue graft.