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Abstrait
Induction of Sulfated Polysaccharides in Spirulina platensis as Response to Nitrogen Concentration and its Biological Evaluation
Abd El Baky H*,Hanaa El Baz KF,EL-Latife SA
Sulfated polysaccharides (SPS) extracted with hot water from Spirulina grown on nutrient medium containing 412 ppm (optimal N2 level) and 45 ppm (limited N2 level) ppm nitrogen were found to be rich in sulfate contents with values 5.02 % and 4.13%), respectively. Monosaccharides content of SPS in both algae cells were analyzed by HPLC and the results showed that: glucouronic acid and galactose were the predominating sugar in all extracts, followed by rhamnose, arabinose, glucose and ribose. The SPS analyzed by FT-IR spectra showed an intense band of absorption at 790-850 cm-1 indicating the presence of sulfate group of polysaccharides. SPS of S. platensis exhibited anticoagulant activities as compared with heparin (standard anticoagulant drug). SPS showed a significant growth inhibit (%) against HepG2 and MCF7 cancer cell lines, with IC50 value ranged between 4.0 and 0.54 g/ml. SPS showed antiviral activity against HSV-1 (standard strain) as a model of DNA virus (as assessed by a plaque reduction test and antioxidant activities toward DPPH. and ABTS.+ radical.