Indexé dans
- Ouvrir la porte J
- Genamics JournalSeek
- Clés académiques
- JournalTOCs
- Infrastructure nationale des connaissances en Chine (CNKI)
- Répertoire des périodiques d'Ulrich
- RechercheRef
- Université Hamdard
- EBSCO AZ
- Répertoire d'indexation des résumés pour les revues
- OCLC - WorldCat
- Publions
- Fondation genevoise pour la formation et la recherche médicales
- Pub européen
- Google Scholar
Liens utiles
Partager cette page
Dépliant de journal
Revues en libre accès
- Agriculture et aquaculture
- Alimentation et nutrition
- Biochimie
- Bioinformatique et biologie des systèmes
- Business & Management
- Chimie
- Génétique et biologie moléculaire
- Immunologie & Microbiologie
- Ingénierie
- La science des matériaux
- Neurosciences & Psychologie
- Science générale
- Sciences cliniques
- Sciences environnementales
- Sciences médicales
- Sciences pharmaceutiques
- Sciences vétérinaires
- Soins infirmiers et soins de santé
Abstrait
Isolation and Characterization of Buffalo Wharton’s Jelly Derived Mesenchymal Stem Cells
Sreekumar TR, Mohd Matin Ansari, Vikash Chandra G and Taru Sharma
Bone marrow and adipose tissue are the most common sources of animal mesenchymal stem cells. However Wharton’s jelly derived stem cells which could be collected non-invasively can be an alternative and good source of stem cells. The objective of present study was to isolate and characterize putative stem cell population from Wharton’s jelly derived cells. Fibroblastoid, plastic adherent cells could be successfully isolated from Wharton’s jelly and expanded in vitro conditions. Cells showed high proliferation ability and stained positively for alkaline phosphatase. Expression profile of surface cell markers and pluripotency markers were analyzed using immunocytochemistry and RT-PCR. Relative transcript abundance of pluripotency markers were analyzed by SYBR green based real time PCR. Karyotyping was done to demonstrate chromosome integrity. When corresponding induction conditions were applied, bWJ-MSCs differentiated into osteogenic, adipogenic and chondrogenic lineages which was confirmed through von Kossa, Oil Red O and Alcian blue staining respectively. In conclusion, buffalo Wharton’s jelly represents a promising source of mesenchymal stem cells with high proliferative and differentiation potential and these cells could provide great scope for multiple regenerative therapies.