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Abstrait
Prevalence of Inhibitor Resistant Beta Lactamase Producing E. coli in Human and Poultry Origin of Bangladesh
Md. Anowar Khasru Parvez, Mahfuza Marzan, Syeda Moriam Liza, Taslin Jahan Mou, Ishrat Jahan Azmi, Md. Shahedur Rahman and Zahid Hayat Mahmud
Human clinical specimens (n=48) and poultry fecal specimens (n=40) were collected from a sub district (Savar) of Bangladesh. Total 25 E. coli were isolated from these specimens. These E. coli were tested for their antibiotic sensitivity against commonly used antibiotics. In addition, to detect the ESBL (extended spectrum beta lactamases) producers, double disk synergy (DDS) test and PCR method were used. Twenty three E. coli were multidrug resistant, i.e. resistant against at least three different groups of antibiotics. DDS method showed all E. coli from poultry specimens and 78% of E. coli from clinical specimens were resistant to amoxicillin-clavulanic acid combination. Thus they are phenotypically confirmed as inhibitor resistant beta lactamase producers. PCR result showed only blaTEMgene in E. coli from poultry specimens. Thus inhibitor resistant type β-lactamase was found to be dominant in E. coli from both types of specimens.