Journal de la science et de la technologie des membranes

Abstrait

Protein Separation from fermentation Broth (E. coli) using polyethersulfone UF and MF membranes

Prafulla G Bansod and VS Sapkal

In this paper, the intracellular E.coli fermentation broth was developed in fermentor. The broths were separated using 0.2μm microfiltration polyethersulfone membranes. The Cells of E.coli was retained in microfiltration membrane, were broken in high pressure homogenization. The cells debris and protein were separated using polyethersulfone 0.2μm PES Microfiltration membranes. In microfiltration, cells of E.coli were rejected and proteins collected in permeate sides. After microfiltration, 30KD Ultra filtration membrane was used to separate proteins. About 91.01 % of the proteins were separated by the ultra filtration polyethersulfone membrane.