Indexé dans
- Ouvrir la porte J
- Genamics JournalSeek
- Clés académiques
- JournalTOCs
- CiteFactor
- Répertoire des périodiques d'Ulrich
- Accès à la recherche mondiale en ligne sur l'agriculture (AGORA)
- Bibliothèque des revues électroniques
- Centre international pour l'agriculture et les biosciences (CABI)
- RechercheRef
- Répertoire d'indexation des revues de recherche (DRJI)
- Université Hamdard
- EBSCO AZ
- OCLC - WorldCat
- érudit
- Catalogue en ligne SWB
- Bibliothèque virtuelle de biologie (vifabio)
- Publions
- Fondation genevoise pour la formation et la recherche médicales
- Pub européen
- Google Scholar
Liens utiles
Partager cette page
Dépliant de journal
Revues en libre accès
- Agriculture et aquaculture
- Alimentation et nutrition
- Biochimie
- Bioinformatique et biologie des systèmes
- Business & Management
- Chimie
- Génétique et biologie moléculaire
- Immunologie & Microbiologie
- Ingénierie
- La science des matériaux
- Neurosciences & Psychologie
- Science générale
- Sciences cliniques
- Sciences environnementales
- Sciences médicales
- Sciences pharmaceutiques
- Sciences vétérinaires
- Soins infirmiers et soins de santé
Abstrait
Silent Mutation: Characterization of its Potential as a Mechanism for Sterol 14α-Demethylase Resistance in Cercospora beticola Field Isolates from the United States
James O Obuya, Anthony Ananga and Gary D. Franc
Sterol
demethylation
inhibitors (DMIs) are considered among the most effective fungicides used to control
Cercospora
leaf spot (CLS), caused by Cercospora beticola Sacc., in sugar beet. Resistance to DMI fungicides has been reported in the C. beticola population from the United States, but the molecular mechanism is not known. It is considered that genetic changes in the C. beticola 14α-demethylase (CbCyp51) gene may be contributing to DMI resistance. The study investigated a silent mutation (GAG to GAA) at codon 170 as a potential mechanism for C. beticola DMI resistance. The CbCyp51 gene was obtained from DMI-sensitive and -resistant isolates, cloned into a plasmid vector, transformed in an isogenic yeast R-1, and tested for DMI sensitivity. Transformed yeast showed low ED50 values (0.02 - 0.09 μg ml–1) as compared to high ED50 values from C. beticola DMI-resistant isolates (21 - 65 μg ml–1). The finding did not support our hypothesis that a silent mutation in the CbCyp51 gene may be associated with C. beticola DMI resistance. Furthermore, genetic analysis of the CbCyp51 gene found no mutation in 2 C. beticola DMI-resistant isolates from the Central High Plains. Further studies will be required to investigate additional mechanisms which have been associated with DMI resistance in fungi. Thus, we could not develop a molecularbased assay for the rapid detection of C. beticola DMI resistance, because no
mutation
was found in the CbCyp51 gene. Currently, fungicide sensitivity assay could be the best method screen for C. beticola DMI resistance.