Indexé dans
- Base de données des revues académiques
- Genamics JournalSeek
- Clés académiques
- JournalTOCs
- Infrastructure nationale des connaissances en Chine (CNKI)
- Scimago
- Accès à la recherche mondiale en ligne sur l'agriculture (AGORA)
- Bibliothèque des revues électroniques
- RechercheRef
- Répertoire d'indexation des revues de recherche (DRJI)
- Université Hamdard
- EBSCO AZ
- OCLC - WorldCat
- Catalogue en ligne SWB
- Bibliothèque virtuelle de biologie (vifabio)
- Publions
- MIAR
- Commission des bourses universitaires
- Fondation genevoise pour la formation et la recherche médicales
- Pub européen
- Google Scholar
Liens utiles
Partager cette page
Dépliant de journal
Revues en libre accès
- Agriculture et aquaculture
- Alimentation et nutrition
- Biochimie
- Bioinformatique et biologie des systèmes
- Business & Management
- Chimie
- Génétique et biologie moléculaire
- Immunologie & Microbiologie
- Ingénierie
- La science des matériaux
- Neurosciences & Psychologie
- Science générale
- Sciences cliniques
- Sciences environnementales
- Sciences médicales
- Sciences pharmaceutiques
- Sciences vétérinaires
- Soins infirmiers et soins de santé
Abstrait
Spectral Analysis and Antibacterial Activity of Methanol Extract of Roots of Echinops echinatus and its Fractions
Muhammad Younus, Farrakh Zia Khan, Sabira Sultana and Hafiz Muhammad Asif
Objective: The root of Echinops echiantus L. (Asteraceae) is reported to have great medicinal value. The aim of the present study was to determine the chemical profile of methanol extract of Echinops echiantus by two different spectral analyses which will be useful for its proper identification when therapeutically used. Isolated compounds and crude methanol extract were screened for antibacterial activity.
Methods: Spectral analysis was done by different analytical methods such as Infrared spectroscopy (FTIR) and Ultraviolet spectroscopy. The in vitro antibacterial activity of E. echinatus root extracts was studied by the hole plate diffusion method against several human pathogenic gram-positive and gram-negative bacteria.
Results: Five compounds were isolated from crude methanol extract using column chromatography and characterized by techniques like Infrared spectroscopy (FTIR) and Ultraviolet spectroscopy. Compounds showed different Rf values ranging from 67 to 9.4. All the five isolated compound and crude methanol extract showed significant antibacterial activity against all strains. However, the maximum activity was shown by compound Ee-4, Ee-1, Ee-3 against Staphylococcus aureus (17.0 mm), (15.3 mm), (12.3 mm) respectively. While Ee-5 give maximum activity against E. coli (13.5 mm).
Conclusion: The result of this study suggested that methanol extract of Echinops echiantus root and its isolated compounds exhibited marked antibacterial activity. Therefore, the results of this study may act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.