Journal de la nanomédecine et de la nanotechnologie

Abstrait

The Cytotoxic Effect of Polyelectrolyte Shells Coated Bacterial Cells on Human Leukemia Cells

M. Borkowska, M. Lyzniak, A. Grzeczkowicz, R. Stachowiak, J. Kawiak, J. Bielecki, B. K. Budziszewska and Ludomira H. Granicka

Encapsulation of cells in polymeric shells allowing for separation of biological material from produced factors may find application in the systems for biological processes regulation. Inadequate efficiency of existing therapeutic anticancer regiments and the rise of multi-drug resistant cancer cells have required investigations into novel anticancer strategies. Enhancement of apoptosis in tumors has been suggested as a new anticancer strategy. Pathogenic microorganisms may have the role as the source of agents for apoptotic therapy.

Modified cells of Bacillus subtilis were encapsulated using layer-by-layer technique within polymeric shells for application in local anti-tumor therapy.

The applied shells were modified with incorporated fullerene derivate to ensure the layers stability and integrity.The impact of modified nano-thin shells coated bacterial cells on human leukemia cells was evaluated in vitro. It was observed that coating with applied polyelectrolyte layers with incorporated fullerenol allowed for bacterial cells functioning during the culture period and the lethal impact on eukaryotic cells was observed.

Applied membrane conformation allowing for functioning of encapsulated microorganisms may be recommended or coating shells for local anti-tumor treatment purposes.