Indexé dans
- Accès en ligne à la recherche en environnement (OARE)
- Ouvrir la porte J
- Genamics JournalSeek
- JournalTOCs
- Scimago
- Répertoire des périodiques d'Ulrich
- Accès à la recherche mondiale en ligne sur l'agriculture (AGORA)
- Bibliothèque des revues électroniques
- Centre international pour l'agriculture et les biosciences (CABI)
- RechercheRef
- Répertoire d'indexation des revues de recherche (DRJI)
- Université Hamdard
- EBSCO AZ
- OCLC - WorldCat
- érudit
- Catalogue en ligne SWB
- Bibliothèque virtuelle de biologie (vifabio)
- Publions
- MIAR
- Commission des bourses universitaires
- Pub européen
- Google Scholar
Liens utiles
Partager cette page
Dépliant de journal
Revues en libre accès
- Agriculture et aquaculture
- Alimentation et nutrition
- Biochimie
- Bioinformatique et biologie des systèmes
- Business & Management
- Chimie
- Génétique et biologie moléculaire
- Immunologie & Microbiologie
- Ingénierie
- La science des matériaux
- Neurosciences & Psychologie
- Science générale
- Sciences cliniques
- Sciences environnementales
- Sciences médicales
- Sciences pharmaceutiques
- Sciences vétérinaires
- Soins infirmiers et soins de santé
Abstrait
The Development and Application of Genetic Markers for Giant Freshwater Prawns Macrobrachium Rosenbergii by Microsatellites
Siripavee Charoenwattanasak, Rakpong Petkham, Aruneepong Srisathapom and Pornthep Niamphithak
This article reports the development of microsatellite primers for giant freshwater prawns (Macrobrachium rosenbergii), using the genome library for this type of prawns and biotinylated probe in isolating six types of DNA fragments with base sequence repeats, namely (AG)10, (TG)10, (CAA)10, (CAG)10, (GAT)10 and (TAC)10. Four clones harboring microsatellites were chosen, namely SH2-9F, SH2-10C, SH2-11D and SH3-11G. Base sequencing found no microsatellite sequences. Four pairs of primers were designed, namely DTLSH 7, DTLSH 8, DTLSH 9 and DTLSH 12, respectively. These primers were tested on the DNA of giant freshwater prawns, and DNA band sizes were found to be 131, 174, 210 and 193 per 205 bp, respectively. The developed microsatellite primers may be used in conjunction with primers for other types of prawns. Genetic diversity checking of giant freshwater prawns in Thailand showed that their population in Thailand may be classified into two groups: one with the closest genetic relationship being those from Khon Kaen, Samut Songkhram and Ang Thong provinces while the other with similar genetic characteristics were those from these three provinces as compared to those from Surat Thani province.