Journal des troubles sanguins et de la transfusion

Abstrait

The Effect of (Non-) Agitating Condition on Agonist Induced-Aggregation of the 48 hours-Stored Platelet Concentrates

Timori NH and Badlou BA

Platelets (PLTs) transfusion and their efficacy in a recipient depend on PLTs quality and quantity pretransfusion. One of the important aspects of treating PLT concentrates (PCs), which has controversial effect on functionality is storage of the PCs in an active metabolic state during prolonged storage either by (gentle-) agitation or by rotations in the different angels.

Study design and Methods: The amount of PCs obtained through double centrifugation methods, as described. We measured ADP, Collagen and ristocetin-induced aggregation after 48 hours of storage in PC kept under continuous agitation (CA6h), and in those in which agitation was stopped in the last 6 hours i.e Without Continuous Agitation for 6 Hours (WCA6h).

Results: In Timori et al. BT 201 we have shown that an interruption of 6 hours on PCs has no deleterious effect on PCs P-selectin, PF4, LDH release, pH, swirling, and count. In this study we focus more in details about different agonist induced-aggregation functions, and their receptors’ (ir-) responsiveness and (de-)sensitivity, signal transduction pathways, of the WCA6h and CA6h stored counterparts. The mean level of aggregation response to collagen, ADP and ristocetin in agitated CA6h versus WCA6hr were 3.49 ± 1.73% vs. 3.46 ± 1.0% (p< 0.962), 4.30 ± 2.7% vs. 3.20 ± 3.9% (p< 0.518), and 79.2 ± 4.4% vs. 66.65 ± 28.55% (p<0.186), respectively.

Discussion: We observed no significant differences between different receptors affected by interruption to respond for firmed aggregates. Thus, a short period of 6 hours non-agitating condition after 42 hours continue agitation in the permeable bags had not deleterious effect on PCs agonist-induced aggregation. Compared to disrupted, continue agitation has no superior effect on old PCs’(ir-) responsiveness and (de-)sensitivity after 48 hours.