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Analysis of Fructose 1,6-Diphosphate in Fermentation Broth Using Ion Chromatography
Jian ming Wang, Ying Xun, Ping Hu, Jin xiu Zhang and Li-an Wang
In this study, a new method for rapid and accurate detection of fructose 1,6-diphosphate (FDP) present in fermentation broth was established using ion chromatography. With an AS11-HC anion column, suppressed conductivity detection, and 50 m mol/L KOH elution, qualitative and quantitative analyses of FDP in the fermentation broth can be completed in 5 min. The minimum detection limit of the method for FDP was 0.032 μmol/L (S/N=3). Within the concentrations ranging from 3.3 to 211.5 μmol/L a significant linear relationship (r=0.9999, p<0.0001), good recovery (99.0%~100.3%), and measurement precision (≤0.04%, n=5) were obtained. The method can qualitatively analyze PO4 3-, fructose-6- phosphate, and glucose 6-phosphate in the fermentation broth simultaneously. The results obtained by this method had no significant difference from those obtained using the enzymatic analysis method.